This translation relates to a patent describing a recombinant insulin biosynthesis method, covering core stages of the technological process, applied microorganisms, purification parameters and conditions for obtaining a pharmaceutically active substance
Translation of a patent describing a recombinant insulin biosynthesis method requires thorough knowledge of biotechnological processes and patent law. The document discloses stages of target protein production using genetically engineered strains, cultivation conditions, isolation techniques and purification procedures. Accurate rendering of terminology related to molecular biology, fermentation and analytical quality control is crucial. Any mistranslation may distort the scope of legal protection and affect commercial exploitation of the invention. A professional translation preserves the logical flow, patent section structure and legal equivalence of claims and descriptions. Such a translation is suitable for international patent filings, novelty examination and intellectual property protection
| Source | Target |
| The present invention relates to a method for producing recombinant human insulin in a host microorganism. The method comprises introducing an expression vector encoding a proinsulin precursor into a bacterial cell, cultivating the transformed cell under controlled fermentation conditions, isolating the expressed protein and converting it into biologically active insulin through enzymatic cleavage and purification steps. The described process allows high yield production with improved stability and reduced impurity content, making it suitable for large scale pharmaceutical manufacturing | Настоящее изобретение относится к способу получения рекомбинантного человеческого инсулина в клетке микроорганизма-хозяина. Способ включает введение экспрессионного вектора, кодирующего предшественник проинсулина, культивирование трансформированной клетки в контролируемых условиях ферментации, выделение экспрессированного белка и превращение его в биологически активный инсулин посредством ферментативного расщепления и стадий очистки. Описанный процесс обеспечивает высокий выход продукта, повышенную стабильность и сниженное содержание примесей, что делает его пригодным для крупномасштабного фармацевтического производства |
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